Fig. 4: Identification of collagens within spongin fibers of H. communis marine demosponge.
From: Discovery of mammalian collagens I and III within ancient poriferan biopolymer spongin
a SDS-PAGE analysis of spongin extracted by method C (see “Methods” and Supplementary Figs. 7–9) indicating the presence of three collagen chains (210723A, 210723B, 210723C). b Identification of collagens I and III in this spongin with Western Blot—two bands present. c Identification of Collagen III in the spongin with Western Blot—one band detected. Sample A—disintegrated spongin (pH 7.1); Sample B—disintegrated spongin (pH 2.3). Both the primary antibodies (Collagen I and III) as well as HRP-conjugated anti-rabbit secondary antibodies were used at dilution 1:1000 (see “Methods”). Merged results for proteins identified by LC-MS/MS in specific bands are given in Table 1. Each investigation was repeated 3 times. Source data are provided as a Source Data file.
