Fig. 4: Conformational change of LarA induced by binding to the NTD.
From: Structural basis for the allosteric activation of Lon by the heat shock protein LarA
a Structure of the NTD-bound LarA (this work, pink) and that of free LarA (blue) predicted by AlphaFold, shown in ribbons in a similar orientation. An illustration of the secondary structure elements of the NTD-bound LarA is shown on top, with the Glu residues located in the loop regions marked. b Superimposition of the core structures of LarA in the NTD-bound and predicted free forms. c Limited proteolysis by the protease Glu-C of LarA in the presence and absence of the NTD. The LarA fragment corresponding to cleavage at the residue Glu82 is indicated by the asterisk. The gel shown is representative of two independent experiments with consistent results. Source data are provided as a Source Data file.
