Fig. 4: Brain-targeting evaluation of BE-ST nanoparticles. | Nature Communications

Fig. 4: Brain-targeting evaluation of BE-ST nanoparticles.

From: Berberine-inspired ionizable lipid for self-structure stabilization and brain targeting delivery of nucleic acid therapeutics

Fig. 4

a Cellular uptake analysis by flow cytometry following DRD3 knockdown with siRNA. b Zeta potential measurements of BE complexed with different organic acids. c Brain-to-liver fluorescence distribution ratio of BE formulated with various organic acids by ex vivo imaging. d In vivo and ex vivo imaging of LNP@DiR distribution 1 h post-injection of BE-organic acid complexes. e Plasma concentration-time profiles of BE and BE-ST (1:5 w/w) in SD rats following intravenous administration. f Time-course in vivo imaging of LNP@DiR distribution at 0.5, 1, 2, 4, and 8 h post-injection. g Ex vivo brain imaging at 1, 2, 4, and 8 h post-administration. h Quantitative analysis of brain fluorescence intensity over time. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined by two-tailed unpaired Student’s t test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; NS not significant. Source data are provided as a Source Data file.

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