Fig. 7: BE-ST enables efficient delivery of small-molecule and macromolecular drugs in vivo. | Nature Communications

Fig. 7: BE-ST enables efficient delivery of small-molecule and macromolecular drugs in vivo.

From: Berberine-inspired ionizable lipid for self-structure stabilization and brain targeting delivery of nucleic acid therapeutics

Fig. 7

a Experimental timeline for cryptococcal meningitis treatment in mice using amphotericin B (AMB). b, c In vivo fluorescence imaging and quantification. Data are presented as mean ± SD (n = 5 biologically independent animals). d Brain tissue fungal colony burden quantification. Data are presented as mean ± SD (n = 6 biologically independent animals). e Kaplan-Meier survival analysis (n = 10 biologically independent animals). f Fluorescence microscopy of eGFP mRNA in vitro transfection. Scale bar, 25 µm. g Three-dimensional visualization of polyA-A2-B13 binding site interactions (PDB ID: 3GIB). h Agarose gel electrophoresis showing BE-loaded mRNA stability. i Three-dimensional hyalinization microscopy of brain tissue following 10 µg eGFP mRNA administration. Scale bar, 1500 µm. Statistical significance was determined by two-tailed unpaired Student’s t test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are representative of three (f, h) and two (i) independent experiments with similar results. Source data are provided as a Source Data file.

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