Fig. 4: Evaluation of the ability of Zn2+-loaded microrobots to promote tendon regeneration in vitro.
From: Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface
A Live/Dead staining of TCs after coculture with the indicated microspheres for 3 and 7 days. B CCK-8 assay of TCs after coculture with the indicated microspheres for 1, 3, 5, and 7 days. C Crystal violet staining of migrated TCs in the Transwell experiment. D Scratch wound healing assays of TCs at 0 and 36 h. E Quantitative analysis of migrated TCs in the Transwell experiment. F Semiquantitative analysis of the migration area in the scratch wound healing assay at 12, 24, and 36 h. G COL-I, COL-III, and TNC immunofluorescence staining (green) of TCs. The cells were costained with DAPI (blue) and phalloidin (red). H The relative mRNA expression of COL-I, COL-III, TNC, and MKX in TCs. I Schematic illustration of Zn2+-loaded microrobots promoting the proliferation, migration, and tenogenesis-related functions of TCs. Data are presented as mean ± SD (n = 3 biologically independent samples). B, F two-way ANOVA with Dunnett’s multiple comparisons test and adjustment applied. E, H one-way ANOVA with Dunnett’s multiple comparisons test and adjustment applied. I created in BioRender. Ding, Z. (2025) https://BioRender.com/p69t917. Source data are listed in the Source Data file.
