Fig. 1: Indirect proximity labeling within PKD-2-carrying EVs identified candidate interactors of the polycystin complex. | Nature Communications

Fig. 1: Indirect proximity labeling within PKD-2-carrying EVs identified candidate interactors of the polycystin complex.

From: Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans

Fig. 1

a Scheme of EV harvest and enrichment, followed by pulldown of candidate interactors biotinylated by TurboID targeted to PKD-2-carrying EVs. b Scheme of male tail sensory rays that release PKD-2 EVs. Each sensory ray of the male tail harbors a ciliated dendritic ending protruding into the environment and releasing PKD-2 EVs from the tip of its sensory cilium. c Fluorescence image of EVs released from the sensory rays carrying PKD-2::GFP and TurboID indicative of successful targeting of the biotin ligase to PKD-2-carrying EVs. See Supplementary Fig. 1 for additional images. d Identified top candidate PKD-2 interactors, validated in subsequent figures. Created in BioRender. Barr, M. (2025) https://BioRender.com/n98b409.

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