Fig. 4: The polycystin complex associates with and recruits a channel-like protein PACL-1 to cilia and EVs.
From: Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans
a Flattened z-stack showing colocalization of PKD-2::mScarlet with PACL-1::GFP in WT cilia with a representative fluorescence profile (n = 12 cilia) (a’) and a cumulative correlation plot (n = 114 cilia) (a”) showing a high level of colocalization in the ciliary tip and shaft regions. Pearson correlation coefficients from a two-sided test are reported alongside unadjusted p-values. For the full dataset that includes all rays, refer to Supplementary Fig. 8. Source Data are provided as SourceData_Main.xls and SourceData_SuppFig8.xls files. b–b’ Quantification showing colocalization of PACL-1::GFP to PKD-2::mScarlet on ciliary EVs (b) with high Pearson correlation (two-sided) coefficients for raw fluorescent intensities (b’), n = 7 animals. The box-and-whiskers plots show the median values where the box covers two quartiles around the median, and the whiskers extend 1.5 quartiles from the median. Source Data are provided as SourceData_Main.xls file. c Flattened z-stacks show that in the lov-1 mutant, PACL-1::GFP remains in the cell bodies and fails to move to dendrites and cilia. d Working model of the molecular mechanism loading PACL-1 to ciliary EVs. LOV-1 is required for PACL-1 ciliary localization. In the lov-1 mutant, cilia produce EVs without PACL-1. A rationale for the PACL-1 channel-like identity is presented in Supplementary Fig. 7.
