Fig. 8: In vivo evaluation of photo-immunotherapy activation mediated by EcN@INX-2.

a Representative flow cytometry plots depicting DCs maturation within tumor tissues following diverse treatments. b Representative flow cytometry plots depicting CD3⁺CD4⁺ helper T cells within tumor tissues following diverse treatments. c Representative flow cytometry plots depicting CD3⁺CD8⁺ cytotoxic T cells within tumor tissues following diverse treatments. d Quantitative analysis of flow cytometric results for mature DCs (n = 3 mice). e Quantitative analysis of flow cytometric results for CD3⁺CD4⁺ helper T cells (n = 3 mice). f Quantitative analysis of flow cytometric results for CD3⁺CD8⁺ cytotoxic T cells (n = 3 mice). g Representative CLSM captures of immunofluorescence staining depicting etco-CRT (red fluorescence) and HMGB1 (green fluorescence) on tumor sections from mice subjected to distinct treatment regimens. Nuclei (blue) were stained using DAPI. (Scale bar: 50 μm). h Representative CLSM captures of immunofluorescence staining depicting CD11c⁺ DCs (green fluorescence), CD4⁺ T (red fluorescence) and CD8⁺ T (green fluorescence) cells on tumor sections from female mice subjected to distinct treatment regimens. Nuclei (blue fluorescence) were stained using DAPI. (Scale bar: 50 μm). Data are presented as mean ± SD (n = 3 mice). For (d, e and f), statistical analyzes were performed using one-way ANOVA. For (a, b, c, g and f), experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.