Fig. 8: In vivo immunotherapeutic effects.
From: Quasi Fe MIL-53 nanozyme inducing ferroptosis and immunogenic cell death for cancer immunotherapy
a Schematic illustration of experimental process of in vivo antitumor immunity. b Flow cytometry analysis of mature DCs of tumors after saline and Q-MIL-53(Fe) treatment. c Flow cytometry analysis of CD4+ T cells and CD8+ T cells of tumors after saline and Q-MIL-53(Fe) treatment (the CD4+ T cells and CD8+ T cells were gated on CD3+ T cells). d, e IF staining of tumor sections for Granzyme B (d) and FOXP3 (e) expression. Images are representative of three biologically independent mice. Scale bar = 200 μm. f Schematic illustration of the experimental process of in vivo immunotherapeutic effects. g The tumor growth curves of 4T1 tumor-bearing mice after indicated treatments. The significance between each of the multiple groups was calculated using one-way ANOVA. h Images of the harvested tumors. i The recorded weight of tumors after different treatments. The significance between each of the multiple groups was calculated using one-way ANOVA. j The recorded weight of 4T1 tumor-bearing mice after indicated treatments. k Photographs of tumors and major organs (heart, liver, spleen, lung, and kidney). l H&E staining of the tumors sections. Scale bar = 100 μm. Images are representative of three biologically independent mice. All data are presented as means ± SD (n = 5 biologically independent animals for g,i,j). Source data are provided as a Source Data file.
