Fig. 6: Reduction of KAT7 HAT activity inhibits the expression of genes associated with procentriole formation.

a Volcano plot showing the mRNA changes upon the depletion of KAT7 in HCT116 cells. Downregulated (green) and upregulated (red) genes (fold change ≥ 1.5) are marked in blue or red, respectively. Adjusted P-value based on benjamini-Hochberg method and fold change was used to draw volcano plot. n = 1 biological replicate. b GO enrichment analysis of the molecular functions and ontology of biological processes with changed genes in (a). Adjusted P-value in GO enrichment analysis based on fisher’s exact test. The color of the circle indicates the adjusted P value. The circle size indicates the number of differentially expressed genes (DEGs). c Heatmaps showing the occupancy of KAT7 in RKO cells centered on peak summits ± 5 kb. The ChIP-seq signal heatmap using a 10 kb window was centered on peak regions. d Genomic distribution of KAT7 binding regions in RKO cells. e Venn diagram showing overlap of KAT7-binding regions and the differentially expressed genes of RNA-seq in KAT7 siRNA cells. f Gene ontology enrichment of overlapped genes in (e) operated by Database for Annotation, Visualization and Integrated Discovery (DAVID). The circle size represents the number of KAT7 target genes associated with each pathway. The circle color gradient represents the P value. P-value based on fisher’s exact test. g HCT116 cells were transfected with non-specific siRNA or KAT7 siRNA, and then were stained with γ-tubulin (red) and DAPI (blue) for immunofluorescence. Scale bar, 2 μm. h The fluorescence intensity of γ-tubulin foci was measured by Image J as the volume of centrosome. Dot plots depict the volume of centrosomes. Centrosome volumes were averaged across the two centrosomes in each cell (control group: n = 49 cells; KAT7 knockdown group: n = 44 cells). Two-tailed unpaired student’s t-test was used. P-values represented in the figure. Error bars represent mean ± SD. i HCT116 cells were treated with etoposide (0, 4 or 8 h, 40 μM), and then cells were harvested to examine the expression of genes encoding procentriole formation proteins by real-time PCR. n = 3 independent biological replicates. One-way ANOVA was used. P-values represented in the figure. Error bars represent mean ± SD. j HCT116 cells were transfected with Myc-Vector, Myc-KAT7-WT or Myc-KAT7-K432R plasmids, and then cells were harvested to examine the expression of genes encoding procentriole formation proteins by real-time PCR. n = 3 independent biological replicates. One-way ANOVA was used. P-values represented in the figure. Error bars represent mean ± SD. Source data are provided as a Source Data file.