Fig. 7: Reduction of KAT7 HAT activity inhibits centriole duplication by impairing the enrichment of H3K14ac at the promoter of genes associated with procentriole formation.

a HCT116 cells were treated with or without etoposide (40 μM, 8 h), and then were stained with centrin-1 (green) and DAPI (blue) for immunofluorescence. A total of 300 cells were captured and representative images are shown. n = 3 independent biological replicates. Scale bar, 2 μm. b The proportion of cells with the indicated numbers of foci were counted. Two-tailed unpaired student’s t-test was used. Exact P-values represented in the figure. Each bar represents mean ± SD. c HCT116 siRNA cells were transfected with Myc-vector, Myc-KAT7-WT or Myc-KAT7-K432R plasmids, and then were stained with centrin-1 (green) and DAPI (blue) for immunofluorescence. A total of 300 cells were captured and representative images are shown. n = 3 independent biological replicates. Scale bar, 2 μm. d The proportion of cells with the indicated numbers of foci were counted. One-way ANOVA was used. Exact P-values represented in the figure. Each bar represents mean ± SD. e HCT116 cells were transfected with Myc-KAT7, and then were stained with centrin-1 (green), Myc (red) and DAPI (blue) for immunofluorescence. Representative confocal images are provided. n = 5 randomly captured images. Scale bar, 2 μm. f–j HCT116 cells were treated with or without etoposide (40 μM, 8 h). A ChIP assay was performed to detect the enrichment of H3K14ac at the promoter regions of genes associated with procentriole formation. n = 3 independent biological replicates. One-way ANOVA was used. P-values represented in the figure. Each bar represents mean ± SD for biological triplicate experiments. f CEP192, g CEP152, h PLK4, i STIL and j SAS6. k–o HCT116 cells were transfected with Myc-vector, Myc-KAT7-WT or Myc-KAT7-K432R plasmids and a ChIP assay was performed to detect the enrichment of H3K14ac at the gene promoter regions of genes associated with procentriole formation. n = 3 independent biological replicates. One-way ANOVA was used and multiple comparisons were applied in these groups. P-values represented in the figure. Each bar represents mean ± SD. (k) CEP192, (l) CEP152, (m) PLK4, (n) STIL and (o) SAS6. Source data are provided as a Source Data file.