Fig. 2: Effect of anti-TG3 antibodies on TG3 activity. | Nature Communications

Fig. 2: Effect of anti-TG3 antibodies on TG3 activity.

From: Enzyme-activating B-cell receptors boost antigen presentation to pathogenic T cells in gluten-sensitive autoimmunity

Fig. 2

a Stimulation of DQ2.5-glia-ω2-specific BW58 T cells by HLA-DQ2.5-expressing A20 B cells in the presence of deamidated or native gluten peptide. The native peptide was either added directly or pre-incubated with active TG3 with or without bound anti-TG3 monoclonal antibodies (mAbs, n = 10) targeting three different epitopes (Ep. 1-3). Dose-response curves (left panel) were used for calculation of EC50 values (right panel). Bar heights indicate means, and error bars indicate SD based on 3 (Ep.1 and Ep.2) or 5 (Ep.3) experiments. b Titratable effect of two epitope 3 mAbs on TG3-mediated deamidation assessed by activation of gluten-specific T cells as in (a). Symbols represent means of two experiments, and error bars indicate range. c SDS-PAGE analysis of proteolytically cleaved TG3 before and after pull-down with anti-TG3 mAbs targeting different epitopes. One of five experiments is shown. Source data are provided as a Source Data file.

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