Fig. 4: MbpR binds DNA sequence specifically. | Nature Communications

Fig. 4: MbpR binds DNA sequence specifically.

From: A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP

Fig. 4

a 3 × FLAG-MbpR bound regions on the L. plantarum WCFS1 chromosome. b Potential MbpR-binding motif identified by MEME-ChIP (https://meme-suite.org/meme/doc/meme.html) based on the ChIP-Seq peaks. c Electrophoretic mobility shift assays (EMSA) analysis of MbpR binding to DNA probes of wild-type and mutated peak 114. d Chromatin immunoprecipitation sequencing (ChIP-Seq) signal density at the transcription start site. TSS: transcription start site. e The number of MbpR-binding sequences located at coding DNA Sequence (CDS), 3’ ends of two genes, and promoter region. The data in (a, c) was performed three times with similar results. f Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) analysis of MbpR bound gene expression in WT and ΔmbpR. Transcription levels are shown as mean ± standard deviations (SD) of 3 biological replicates. Error bars indicate the SD. Dots represent individual data. Statistical significance was analyzed using one-way Analysis of Variance (ANOVA) with Tukey’s multiple-comparison test. Source data are provided as a Source Data file.

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