Fig. 2: SR-A3 deletion induces hypertriglyceridemia, hyperglycemia, and hepatic steatosis in male hamsters on a chow diet. | Nature Communications

Fig. 2: SR-A3 deletion induces hypertriglyceridemia, hyperglycemia, and hepatic steatosis in male hamsters on a chow diet.

From: SR-A3 suppresses AKT activation to protect against MAFLD by inhibiting XIAP-mediated PTEN degradation

Fig. 2

a Schematic representation of 12 week-old male WT and SR-A3−/− hamsters fed a CD for 8 weeks and then fasted for 16 h before sacrifice. b, c Body weight (b), plasma triglyceride (TG), total cholesterol (TC), and non-esterified fatty acid (NEFA) (c) (n = 7/group). d Plasma lipoprotein profile analysis by fast protein liquid chromatography (FPLC) (n = 7/group). e Determination of plasma lipoprotein lipase (LPL) activity (n = 7/group). f Analysis of very-low-density lipoprotein (VLDL) secretion rates (n = 7/group). g Determination of plasma TG levels after olive oil gavage (n = 6/group). h Oil red O staining of jejunal epithelium after olive oil gavage. (WT: n = 5; SR-A3−/−: n = 7). Scale bars, 200 μm. i Plasma glucose level at sacrifice (n = 7/group). j Plasma insulin level was measured 2 h after glucose i.p. injection (2 g/kg) (n = 5/group). k Glucose tolerance test (GTT) and area under curve (AUC) quantification (n = 6/group). l Insulin tolerance test (ITT) and AUC analysis (n = 6/group). m Representative images of gross morphology, Hematoxylin and Eosin (HE), oil red O, and sirius red staining of the livers at sacrifice (n = 7 /group). Scale bars, 100 μm for HE and oil red O, 200 μm for sirius red. n The ratio of liver to body weight (BW), and quantifications of oil red O and sirius red staining (n = 7/group). o Plasma ALT and AST levels (n = 7/group). p Liver TG and TC content (n = 7/group). q PCA analysis of liver lipidome from (a) (n = 3/group). r Three significantly increased lipid species in the livers analyzed by targeted lipidome (WT: n = 4; SR-A3−/−: n = 3). s, t Top 10 differentially changed species in liver triglyceride (TAG), diglyceride (DAG), free fatty acid (FFA) (n = 3/group). u Relative expression levels of genes in livers from (a) (WT: n = 7; SR-A3−/−: n = 8). Data were presented as the mean ± SEM. Statistical significance was determined by unpaired two-sided Student’s t-test (b, c-middle/right, i–k-right, n-middle, o-right, p-right, u), or two-sided Mann–Whitney test (c-left, e, l-right, n-left/right, o-left, p-left) or two-way ANOVA (f–k-left, l-left) with Bonferroni multiple-comparison analysis. Source data are provided as a Source Data file.

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