Fig. 5: eIF4A1/2 is rapidly excluded from the reforming nucleus. | Nature Communications

Fig. 5: eIF4A1/2 is rapidly excluded from the reforming nucleus.

From: The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation

Fig. 5

A eIF4A1/2 immunostaining (green in overlay) of HeLa cells stably expressing H2B-mCherry (magenta in overlay) at mitotic different stages, representative for 4 independent experiments. Scale bars: 10 µm. B eIF4A1/2 immunostaining (green in overlay) of interphase HeLa cells stably expressing H2B-mCherry (magenta in overlay), untransfected or transfected with 20 nM control or a combination of eIF4A1 and eIF4A2 siRNA oligos for 72 h, representative for 4 independent experiments. Scale bars: 10 µm. C Fixed samples of different mitotic stages of HeLa cells expressing H2B-mCherry (magenta in overlay) and GFP or eIF4A1-EGFP (green in overlay), representative for 2 independent experiments. Scale bars: 10 µm. D Fixed samples of interphase HeLa cells expressing H2B-mCherry (magenta in overlay) and GFP or eIF4A1-EGFP (green in overlay), representative for 2 independent experiments. Scale bars: 10 µm. E Time-lapse images of H2B-mCherry HeLa cells expressing eIF4A1-EGFP, the corresponding ATPase deficient mutant eIF4A1 E183Q or the RNA-binding deficient R362Q mutant, EGFP, eIF4A2-EGFP, eIF4A3-EGFP, EGFP-eIF4B, EGFP-eIF4G1 or EGFP-eIF4H, representative for 3 independent experiments per construct. Time is normalized to the first anaphase frame. Scale bars: 10 µm.

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