Fig. 1: Cryo-EM structures of Mip1 captured in the process of DNA strand separation.
From: Structural basis for intrinsic strand displacement activity of mitochondrial DNA polymerase
A A schematic representation of the domain organization of Mip1 and its key structural elements. B Assembly of the strand displacement complex. The DNA template strand (here and throughout) is shown in blue, the primer in red, and the non-template strand in cyan. The incoming nucleotide is in yellow. C The cryo-EM density map of Mip1 (Structure I). D–G De novo models of Mip1 (Structures I-IV). Important structural elements involved in strand separation are highlighted. Fingers – pink, exonuclease domain – light yellow, thumb – orange, catcher loop - wheat, SD-loop – magenta, wedge helix – teal, joist– slate. H Downstream DNA bending by Mip1 during strand separation.
