Fig. 4: BBB-crossing ability of ICG-Gd-Den@DC-G16-11 in vitro. | Nature Communications

Fig. 4: BBB-crossing ability of ICG-Gd-Den@DC-G16-11 in vitro.

From: GCN5-targeted dual-modal probe across the blood-brain barrier for borders display in invasive glioblastoma

Fig. 4

a Illustration of the chip design, which includes a medium-storage layer, an upper chamber for cell seeding, a PET membrane separating the upper and lower layers, and a lower chamber for cell seeding. b TEER measurement of the BBB model fabricated using microfluidic chips. c Immunofluorescence staining for the expression of the tight junction protein ZO-1. Blue: nuclei stained with DAPI; green: FITC-labeled ZO-1(Secondary antibody). d Quantification of fluorescence intensity in the upper and lower chambers of the BBB model for ICG-Gd-Den@DC-G16-11 and ICG-Gd-Den probes, and statistical analysis of the penetration rate of ICG-Gd-Den@DC-G16-11 and ICG-Gd-Den probes in the microfluidic chip BBB model. e Schematic illustration of the modes of transport for probes across the blood-brain barrier. Data in (d) are presented as mean values ± SD. The p value in (d) were determined by unpaired two-tailed Student’s t-test. **p < 0.01.

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