Fig. 7: Evidence of miR-503 and MKNK1 in aging-associated pancreatitis changes in the Chinese population. | Nature Communications

Fig. 7: Evidence of miR-503 and MKNK1 in aging-associated pancreatitis changes in the Chinese population.

From: Endocrine-exocrine miR-503-322 drives aging-associated pancreatitis via targeting MKNK1 in acinar cells

Fig. 7

A Representative images of H&E and Masson staining of pancreatic sections from the young adult (YA) and the elderly adult (EA); quantitation of collagen volume fraction. The dashed area indicates acini. n = 10 and a total of 30 photographs were taken for statistical analysis. B Representative images of immunofluorescence staining of PCNA (green) in pancreatic sections and counted the number of PCNA-positive cells. n = 10. Arrows indicate proliferating acinar cells. n = 10 and a total of 30 photographs were taken for statistical analysis. C In situ hybridization of miR-503 (40 nM) in young and elderly pancreatic sections. Scramble-RNA was negative reference (40 nM), and U6 was positive reference (0.1 nM). The dotted line indicates islet, and the solid line is exocrine. n = 3 independent experiments. D Representative images of immunofluorescence staining of MKNK1 (green) and amylase (red) in pancreatic sections from young and elderly people and quantitation of amylase and MKNK1 mean fluorescence intensity. n = 10. E Serum amylase assay of the young adult (YA), the elderly adult (EA) and the elderly adult with diabetes (EA + DM). YA group, n = 65. EA group, n = 65. EA + DM, n = 30. F MiR-503 concentration in human serum of YA, EA and EA + DM. YA group, n = 45. EA group, n = 45. EA + DM, n = 30. G Correlation analysis of amylase levels of human serum and age. Each point represents one people (n = 160). Correlation coefficient (R) and p value from simple linear regression are shown. H Correlation analysis of miR-503 concentration in human serum and serum amylase levels. Each point represents one people (n = 120). Correlation coefficient (R) and P value from simple linear regression are shown. Box plots with centerline = median, box = 25th–75th percentile, and whiskers = 5th–95th percentile, outliers = open circles (A, B, D–F). Data are means ± SEM. Data were analyzed using unpaired Student’s t tests (A, B, D), one-way ANOVA with Tukey test (E, F) or Correlation analysis (G, H). Source data are provided as a Source Data file.

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