Fig. 2: mRNAs localize to Mushroom Body γ axons.
From: Axonal RNA localization is essential for long-term memory
Confocal images of smFISH signals (magenta) obtained from whole-mount adult brains using anti-rpl15 (A), gfp (B–D), and His3.3B (E) probe sets. The population of MB γ axons (ax) is labeled in green in the bottom overlay images. Boxed areas correspond to single confocal sections of MB γ neuron cell bodies (cb). In (B, C) GFP protein-trap knock-in lines were used to detect lk6 and rols mRNA distribution. In (D) control flies in which GFP is not expressed were used to validate gfp probe specificity. MB γ axons were labeled using the 201Y-Gal4 driver in combination with either UAS-cGFP (A, E) or UAS-CD8-RFP (B–D). Asterisks indicate signals corresponding to the soma of neighboring neuronal populations. Scale bar: 10 μm. F Graph plotting the correlation between the number of smFISH spots in MB γ cell bodies (x-axis) vs axons (y-axis). Data points correspond to mean values and error bars to SEM. Samples collected from two (rpl23, His3.3B, Serca, camk2, rols, csas) to three (cg2852, lk6, rpl15, rpl24-like) biological replicates, were quantified for each condition. Source data are provided as a Source Data file.
