Fig. 3: Identification of Imp mRNA targets localizing to MB γ axons.
From: Axonal RNA localization is essential for long-term memory
A Overlapping the data sets from Imp iCLIP (bulk; RNAs with at least a significant peak supported by at least 5 uniquely mapped cDNAs), Imp RIP (MB γ neuron-specific; RNAs enriched with a log2FC > 1) and synaptosome (bulk; RNAs with a log2FC > 0) led to the identification of candidate Imp mRNA targets that may localize to the axons of MB γ neurons. B Quantification of the number of smFISH spots in MB γ axons for the candidate Imp-bound mRNAs analyzed. Three to four biological replicates were performed, and data from each replicate was labeled with different colors. Bars represent mean values. pabp mRNA distribution was analyzed using a GFP protein-trap knock-in line. Four outlier data points (two for arc1 and two for eiF4A) were omitted from the graph but considered to calculate the mean. Numbers of brains analyzed: 24 (arc1), 21 (eIF4A), 21 (cg15098), 22 (pabp-gfp), 19 (prof), 22 (eIF4E-1), 18 (act5C), 22 (bnb), 18 (CRC). Confocal images of smFISH signals (magenta) obtained from whole-mount adult brains using anti-eIF4A (C) and anti-profilin (D) probe sets. The population of MB γ axons (ax) is labeled in green in the bottom overlay images. MB γ axons were labeled using the 201Y-Gal4 (C’) or VT44966-Gal4 (D’) drivers in combination with UAS-CD8-RFP (C’) or UAS-CD8-GFP (D’). Asterisks indicate signals corresponding to the soma of neighboring neuronal populations. Scale bar: 10 μm. Source data are provided as a Source Data file.
