Fig. 4: The 3′UTR of Imp mRNA targets mediate binding and promote axonal localization.
From: Axonal RNA localization is essential for long-term memory
A Profiles of the Imp iCLIP (top, two replicates) and RNA-seq (middle, three replicates) signals along the profilin gene regions. Profiles were generated using the clipplotr tool76 and its smoothening function. Intronic and exonic sequences are represented at the bottom by single lines and boxes, respectively (large boxes for coding exons and smaller boxes for UTRs). B Metatranscript profile representing the distribution of Imp iCLIP peaks along a reference transcript. Significant peaks were mapped onto a virtual common transcript based on their relative position within each mRNA. C Numbers of gfp smFISH spots detected in MB γ axons after expression of UAS-driven gfp-3′UTR reporters with the 201Y-Gal4. gfp-SV40 3′UTR transcripts were used as a negative control. Two to three biological replicates were performed, and data color-coded based on the replicate they belong to. Bar graphs and error bars represent, respectively, for each mRNA, the average and SEM of all combined data points. *, P < 0.05; ***, P < 0.001 (Kruskall–Wallis with Dunn’s post-tests). Exact P values: 0.0097 (gfp-lk6 3′UTR), <0.0001 (gfp-pabp 3′UTR), 0.0377 (gfp-prof 3′UTR), 0.0113 (gfp-act5C 3′UTR). Numbers of brains analyzed: 11 (gfp-lk6 3′UTR), 14 (gfp-pabp 3′UTR), 14 (gfp-prof 3′UTR), 12 (gfp-act5C 3′UTR), 8 (gfp-SV40 3′UTR). D mRNA localization of gfp-pabp 3′UTR transcripts expressed using the 201Y-Gal4 driver. Top: schematic representation of the reporter analyzed. Middle: smFISH signal (magenta) obtained using an anti-gfp probe set. Asterisks point to probe aggregates. Bottom: overlay between GFP protein signal (green) and gfp RNA signal (magenta). Scale bar: 10 μm. Source data are provided as a Source Data file.
