Fig. 6: mRNA localization to MB γ axons, but not RNA binding, is altered in imp-Δ PLD mutants.

A Correlation plot showing the normalized counts of 3′UTR-mapped reads obtained for individual RNAs in wild-type (x-axis) and Imp-ΔPLD (y-axis) iCLIP experiments. B Profiles of the Imp wild-type and Imp-ΔPLD iCLIP signals (top, two replicates for each condition) and corresponding input RNA-seq signals (middle, three replicates for each condition) along the profilin gene region. Profiles were generated using the clipplotr tool⁷⁶ and its smoothening function. Intronic and exonic sequences are represented at the bottom by single lines and boxes, respectively (large boxes for coding exons and smaller boxes for UTRs). C, D Confocal images of arc1 smFISH signals (magenta) obtained in control (left) and imp-ΔPLD (right) contexts. The population of MB γ axons (ax) is labeled in green in C’ and D’ using the 201Y-Gal4 driver and UAS-cGFP. Scale bar: 10 μm. E Normalized numbers of smFISH spots detected in MB γ axons of wild-type and imp-ΔPLD mutants. Two to four biological replicates were performed, and the mean value of each is indicated as a triangle. Data points were color-coded based on the replicate they belonged to. 15098 stands for cg15098. **, P < 0.01; ***, P < 0.001 (Two-tailed Mann–Whitney tests). n.s. stands for not significant. Two outlier data points (one for arc1 and one for pabp) were omitted from the graph (but considered to calculate the mean and to perform the statistical tests). Exact P values: <0.0001 (arc1), 0.0058 (eIF4A), 0.0008 (cg15098), <0.0001 (prof), 0.0035 (act5C), 0.0719 (lk6), 0.0902 (crc), 0.9330 (bnb), 0.4527 (eIF4-E1), 0.1925 (pabp). Numbers of brains analyzed for control and imp-ΔPLD conditions respectively: 24 and 25 (arc1), 21 and 19 (eIF4A), 21 and 20 (cg15098), 19 and 20 (prof), 20 and 25 (act5C), 24 and 22 (lk6), 18 and 19 (crc), 22 and 19 (bnb), 22 and 22 (eIF4-E1), 22 and 17 (pabp). Source data are provided as a Source Data file.