Fig. 6: Model comparing Cas12a and SpCas9.
From: Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates
A Cartoons of R-loop states and the effects of mismatches and torque on R-loop state transitions. Arrows represent effects of perturbations due to mismatches or negative torque. B Landscapes of Cas12a orthologs in comparison to SpCas9 with FM guides on relaxed (0 twist) and negatively supercoiled (−5 twist) DNA molecules. Cas12a landscapes are as in Fig. 5A–D, reproduced here for comparison with Cas9. The Cas9 landscapes are adapted from ref. 45.
