Fig. 5: CBP stabilizes pioneer factors binding on chromatin through a possible coiled coil interaction. | Nature Communications

Fig. 5: CBP stabilizes pioneer factors binding on chromatin through a possible coiled coil interaction.

From: Chromatin landscape at cis-regulatory elements orchestrates cell fate decisions in early embryogenesis

Fig. 5

a Heat map of active ZGA genes identified by GRO-seq, clustered by their dependency on the pioneer factor Zelda (Zld-dep) or neither regulated by Zelda or H2A.Z (H2A.Z negative)23 Signal is centred around +/− 2 kb from TSS. Sorting is based on H3K27ac levels before cycle 9 and shows CUT&Tag signal distribution of this mark at three developmental time points and signal distribution of CBP, Zelda and GAF at ZGA in both wild-type and CBP-KD at cycle 14. b CUT&Tag signal of CBP and Zelda at tll locus, where both factors are bound during cycle 14 in wild-type embryos. The highlighted region indicates the localization of the Zelda motif used to predict the possible structure of binding between Zelda and CBP. c Alphafold3102 predicted structure and interaction between CBP and Zelda. Secondary structures are predicted by Alphafold3 and relaxed by molecular dynamics simulations of the DBD or coiled coil regions of CBP and Zelda. The amino acid ID is indicated from N terminus to C terminus. The red and green lines sketch the protein structure of CBP and Zelda, respectively. DNA of tll locus where Zelda motif is present is coloured in blue.

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