Fig. 6: CBP depletion impacts RNA Polymerase II dynamics by increasing its pausing state at zygotically transcribed genes. | Nature Communications

Fig. 6: CBP depletion impacts RNA Polymerase II dynamics by increasing its pausing state at zygotically transcribed genes.

From: Chromatin landscape at cis-regulatory elements orchestrates cell fate decisions in early embryogenesis

Fig. 6

a Profile plot and heat map of active ZGA genes identified by GRO-seq23, classified as Zelda-dependent (Zld-dep), Zelda-independent (Zld-independent) according to101, or non-transcribed genes (GRO-seq negative). Signal is centred around −300 bp/+5 kb from TSS. Sorting is based on GRO-seq levels and shows CUT&Tag normalized signal distribution of RNAPII-S5P and -S2P, respectively initiated and elongated isoforms of RNA Polymerase II, in both wild-type and CBP-KD at cycle 14. b Pausing index of RNAPII-S5P at Zelda-dependent, Zelda-independent and GRO-seq negative genes in both wild-type and CBP-KD condition. Boxes center refers to mean, lower and upper quartiles (Q1 and Q3, respectively). Whiskers, 1.5 × IQR below Q1 and above Q3. Two-sided Mann–Whitney U test. Zelda-dependent genes, P = 6.010 × 10−08; Zelda-independent genes P = 3.141 × 10−35. n = 2 biological replicates per condition. c Genome browser snapshot of Zelda-dependent (tll) or Zelda-independent (sna) loci. CUT&Tag, nuclear RNA-seq and aggregated scATAC-seq for germ layers in wild-type (WT) and CBP-KD data are displayed. Promoter region is highlighted by a blue box, enhancer region is highlighted by a green box.

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