Fig. 7: Multiomic dynamics of germ layer specification during ZGA in Drosophila embryos. | Nature Communications

Fig. 7: Multiomic dynamics of germ layer specification during ZGA in Drosophila embryos.

From: Chromatin landscape at cis-regulatory elements orchestrates cell fate decisions in early embryogenesis

Fig. 7

a Schematic of the 10× Multiome (scRNA-seq + scATAC-seq) profiling of the Drosophila ZGA embryo. b Simultaneous dissection of the chromatin landscape and transcriptomic state from each single nucleus provide a comprehensive overview of the dynamics shaping every cell of the embryo. Differential enrichment of H3K27me3 and H3K27ac at the same loci can shape different transcriptomic outputs by coordinating enhancer activation or repression, providing the blueprint for gastrulation and progression in development. Chromatin factor depletion affects enhancer regulation in two ways: depletion of maternal E(z) and H3K27me3 activate aberrant enhancers across cell-types, causing ectopic gene expression. Instead, CBP loss causes a massive block of differentiation given by the halted transcription at the pre-ZGA stage, while chromatin accessibility can still progress. In both scenarios, embryogenesis cannot be completed. E, Enhancer. P, Promoter. Adapted from an image created in BioRender. Cardamone, F. (2025) https://BioRender.com/s77z537.

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