Fig. 4: Identification of ATs that catalyse acylation in toosendanin/isotoosendanin biosynthesis. | Nature Communications

Fig. 4: Identification of ATs that catalyse acylation in toosendanin/isotoosendanin biosynthesis.

From: Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species

Fig. 4

a Reactions for compounds 1 to 2 and compounds 3 to 4 catalysed by MaAT8824 and MaAT1704. b LC-MS chromatograms of the standard, the control samples and the reaction mixtures for MaAT8824 and MaAT1704 using acetyl-CoA as the acetyl donor and compound 1 as the substrate. c, d LC-MS and LC-MS/MS analyses of reaction product 2 for MaAT8824 and MaAT1704 in positive ion mode; e LC-MS chromatograms of the standards, the control samples and the reaction mixtures for MaAT8824 and MaAT1704 using acetyl-CoA as the acetyl donor and compound 3 as the substrate. f, g LC-MS and LC-MS/MS analyses of reaction product 4 for MaAT8824 and MaAT1704 in positive ion mode. h–k The contents of the four compounds in the four tissues of the two plants. The data are presented as the means ± SDs (n = 3 biological replicates). Source data are provided as a Source Data file.

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