Fig. 4: Defined centrosomal organization of γ-TuRCs. | Nature Communications

Fig. 4: Defined centrosomal organization of γ-TuRCs.

From: Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC

Fig. 4

a Left: Tomographic slice depicting a centrosome in vitrified sections of cells (n = 14). Example γ-TuRCs are highlighted. Scale bar: 100 nm. Right: 3D rendering of the tomogram with MTTs depicted in green and localized γ-TuRCs in yellow. N = 3 biologically independent experiments. b Distribution of center-to-center distances between MTT B-tubules and luminal (negative distance) and pericentriolar (positive distance) γ-TuRCs, respectively, in n = 15 centrioles. c U-ExM of centrioles stained against α-tubulin (gray) and γ-tubulin or NEDD1 (red) illustrating γ-TuRC distribution in centrosomes. Scale bars: 200 nm. N = 3 biologically independent experiments. d Preferred spatial arrangement of lumenal γ-TuRCs with respect to the nearest MTT. A-, B-, and C-tubules of the MTT are indicated. Source data are provided as a Source Data file.

Back to article page