Fig. 2: pPho4 NES binds to the central concave surface of Msn5.
From: Phosphate-dependent nuclear export via a non-classical NES class recognized by exportin Msn5
a Msn5 bound to pPho4112-134 (wheat cartoon; regions 2–4 labeled). Msn5 electrostatic surface potential (red to blue: -10 to 10 eV; same in (d)). b The pPho4 NES sequence (regions 1-4 labeled) with Msn5 contacts represented by yellow, pink, and black asterisks - side chain polar, main chain polar and hydrophobic contacts respectively, blue dotted lines - intra-chain polar. Residues in olive box were not modeled. c Details of pPho4 NES regions 2–4, with contacts shown as dotted lines in same color scheme as (b). d State 1 map of Msn5-RanGTP-pPho4FL (right) colored as in the docked Msn5-RanGTP-pPho41-200 structure (left, in the same orientation, colored as in (a), pPho4 in spheres). Extra continuous density N-terminal of the pPho4112-134, likely occupied by Pho4 NES region 1, is colored in olive. e FP titration of Msn5 into mNeonG-Pho43-200 with excess RanGTP. Residues 102-107 (NES region 1) were mutated to GGSGGS or GKKGKK. Data points are mean ± s.d. from triplicates, line represent 1-site binding and fitting residuals are plotted below. Source data are provided as a Source Data file.
