Fig. 2: Knockout of NETs involved in anchoring heterochromatin at the nuclear envelope does not affect the peripheral localization of MyoD1.
From: MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers
a Immunoblot analysis of total cell extracts of proliferating wildtype and knockout C2C12 clones using antibodies to the indicated antigens. Normalized radial distance of MyoD1 to the nuclear border in wildtype C2C12 myoblasts versus cells with (b) single knockouts, and (c) double or triple knockouts of indicated proteins. H3K9me2-marked heterochromatin layer is shown as gray-shaded bar. Dotted line indicates that experiments were performed separately using slightly different laser settings. nWt = 208, nemerin KO = 333, nLAP2β KO = 140; nWt = 141, nLA/C KO = 403, nLBR KO = 283; nWt = 722, nLA/C&LBR KO = 453, nLA/C, LBR&Emd KO = 300. *pEmd KO = 0.02, remaining p values are non-significant (p > 0.05; two-sample, two-tailed KS test, Hochberg correction for multiple testing). Numeric values displayed on violin plots represent median values. LA/C lamin A/C, Emd emerin. d Localization of emerin assessed by immunofluorescence microscopy in control and lamin A/C-LBR double knockout myoblasts. Scale bars, 10 μm. Images processed with Fiji. Source data are provided as a Source Data file.
