Fig. 4: Identification of nuclear envelope anchors for MyoD1.
From: MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers
a Schematic overview of the strategy for generating multiple protein knockout cell lines. MyoD1 reporter C2C12 myoblasts constitutively expressing Cas9 were transfected with three synthetic sgRNAs targeting the gene of interest. Depletion of various candidate proteins was performed consecutively. b Detection of normalized radial distance of MyoD1 to periphery in proliferating myoblasts following depletion of indicated protein(s). Violin plots were generated from at least 450 data points. nWt = 722, n4KO = 454, n3KO = 898, n2KO = 685, nNET39 KO = 500; nTmem38a KO = 571, nWFS1 KO = 517. ***p4KO = 2 × 10−4, ****p3KO = 3.2 × 10−6, ****p2KO = 7.9 × 10−5, pNET39 KO = 0.6; ***pTmem38a KO = 2 × 10−4, ****pWFS1 KO = 1 × 10−6 (two-sample two-tailed KS test, Hochberg correction for multiple testing). Numeric values displayed on the violin plot represent median values. Source data are provided as a Source Data file.
