Fig. 6: WFS1 associates with the cis-regulatory core enhancer region of the MyoD1 gene.
From: MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers
a Integrative Genomics Viewer (IGV) tracks of the mouse genomic region (mm10) containing MyoD1 and its regulatory region (35 kb upstream) outlined by a gray box. Included are WFS1 ChIP tracks (RPKM, reads per kilobase per million) following sonication for 30 cycles, WFS1 peak regions identified by MACS, constitutive LAD regions (cLADs), ChIP-seq signal tracks of histone modification H3K4me3, H3K27ac, H3K4me1, H3K27me3 and H3K9me3, RNA polymerase II (Pol II) ChIP-seq signal track, enhancer predictions and gene annotations from NCBI reference sequence database (RefSeq) gene track. Known cis-regulatory regions of MyoD1 are shown at the bottom (drawing not to scale). CE core enhancer, DRR distal regulatory region, PRR proximal regulatory region. b Graph showing reads per kilobase per million (RPKM) score of WFS1 ChIP signal within and flanking the core enhancer region of MyoD1 (green line). Red-shaded area corresponds to coordinates of the closest WFS1 MACS peak. c Table showing relevant transcription factor binding sequence motifs identified in WFS1 ChIP-seq peaks by motif enrichment analysis using the HOMER algorithm for known and de novo motifs. p values are indicated. The common consensus, CAGCTG, is a canonical E-box sequence bound by basic helix-loop-helix (bHLH) transcription factors. Motif enrichment p-values were calculated using cumulative binomial distribution (sampling with replacement).
