Fig. 7: WFS1 binds to open, accessible, and active gene regulatory sequences genome-wide.
From: MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers
a Average binding profiles and heat map showing enrichment of WFS1 (log2[ChIP/input]) (30 sonication cycles) on ATAC-seq, H3K27ac, H3K4me1, H3K4me3 and H3K27me3 ChIP-seq peaks ±3 kb from center. Heat maps are ranked according to WFS1 enrichment in descending order. b Degree of overlap (%) of WFS1 MACS peaks with candidate cis-regulatory elements from ENCODE (left). Overlap with randomized peaks is shown as control (right). dELS distal Enhancer-Like Sequences, pELS proximal Enhancer-Like Sequences, PLS Promoter-Like Sequences. c Classification of genes associated with predicted WFS1-bound enhancers based on their expression status in mouse myoblasts. Minimum average expression threshold to classify a gene as expressed is 5 reads in at least two replicates. d Average binding profiles and heat maps displaying enrichment of ATAC-seq, H3K27ac, H3K4me1, H3K4me3, and H3K27me3 ChIP-seq signals on WFS1-bound enhancer regions ± 3 kb from center. Heat maps are ranked in descending order of ChIP-seq signal intensity.
