Fig. 4: Dependence of platelet kinetics and morphology on reaction conditions.

A Unimer dependence. Unimer (PCL₄₅:PCL₄₅-b-PDMA₃₄₈, 1:1, w:w) concentration was varied from 0.53, 1.05, 2.10 to 3.15 μM (5, 10, 20 to 30 μg mL⁻¹). Seed concentration was fixed at 0.13 nM (0.005 μg mL⁻¹). The effective rate constants (\({k}^{{\prime} }\)) extracted from the fitting for each unimer concentration (from low to high) are 0.032, 0.042, 0.058, and 0.066 s⁻¹, respectively. The R² values for the fits at each concentration (from low to high) are as follows: 0.9919, 0.9437, 0.9331 and 0.9429, respectively. B Seed dependence. Seed concentration was varied from 0.25, 0.38, 0.88 to 1.13 nM (0.01, 0.015, 0.035 to 0.045 μg mL⁻¹). Unimer concentration was fixed at 6.31 μM (60 μg mL⁻¹). The effective rate constants (\({k}^{{\prime} }\)) extracted from the fitting for each seed concentration (from low to high) are 0.139, 0.131, 0.170, and 0.218 s⁻¹, respectively. The R² values for the fits at each concentration (from low to high) are as follows: 0.9529, 0.9366, 0.7911 and 0.7983, respectively. C Solvent dependence. THF volume fraction was varied from 0, 1, 3 to 5%. Unimer and seed concentrations were fixed at 2.10 μM and 0.38 nM (20 and 0.015 μg mL⁻¹), respectively. The effective rate constants (\({k}^{{\prime} }\)) extracted from the fitting for each THF concentration (from low to high) are 0.124, 0.123, 0.057, and 0.015 s⁻¹, respectively. The R² values for the fits at each concentration (from low to high) are as follows: 0.7942, 0.4762, 0.8333 and 0.9703, respectively. Error bars in A, B, C represent the standard deviations of the platelet area distribution, calculated from 100 platelets (n = 100) for each time point. D, E, F are the temporal evolution of the ratio between long (L₁) and short axis (L₂) (L₁: L₂, aspect ratio). Error bars in D, E, F represent the standard deviations of the platelet aspect ratio distribution, calculated from 100 platelets (n = 100) for each time point. G, H, I are the distribution of the length of the long (L₁) and short axis (L₂) for the 100 individual platelets (n = 100) analyzed in A–C.