Fig. 5: Multi-annulus platelet formation kinetics.

A Schematic illustration of 4-annulus platelet formation using varied unimer composition via living CDSA. B Montage of iSCAT images during 4-annulus platelet growth (scale bar: 3 μm). 50 μL of 2.51 nM (0.1 μg mL⁻¹) seed solution was spin-coated onto the surface twice, and 4-annulus platelets were formed via alternating addition of PCL₄₅:PCL₄₅-b-PDMA₃₄₈ and PCL₄₅ unimer solutions at concentrations of 0.18, 0.39, 0.35 and 0.77 μM (1.67, 2.08, 3.33 and 4.17 μg mL⁻¹) for each respective annulus. C Size evolution of a 4-annulus platelet. D AFM image of a 2-annulus platelet (scale bar: 3 μm). 50 μL of 2.51 nM (0.1 μg mL⁻¹) seed solution was spin-coated onto the surface, 0.18 μM (1.67 μg mL⁻¹) PCL₄₅:PCL₄₅-b-PDMA₃₄₈ mixtures (PCL concentration: 0.15 μM, 0.83 μg mL⁻¹) in methanol was added to form the first annulus. 0.15 μM (0.83 μg mL⁻¹) PCL₄₅ was then added. E Height profiles corresponding to the cross-section drawn in D. F Montage of iSCAT images during 3-annulus platelet growth (scale bar: 2 μm). 50 μL of 2.51 nM (1.67 μg mL⁻¹) seed solution was spin-coated onto the surface, the PCL₄₅:PCL₄₅-b-PDMA₃₄₈ unimer mixtures were added at concentrations of 0.44, 0.59, and 0.87 μM (4.2, 5.6 and 8.3 μg mL⁻¹) for each annulus. G Mean size evolution of 3-annulus platelets. Rate constants extracted from the fitting for each annulus are 0.017, 0.024, and 0.030 s⁻¹, respectively.