Fig. 4: Phosphoproteomic analysis in the striatum revealed a unique pattern of phosphorylation after a low dose of SKF 38393.

a Heat map of the 50 most variable phosphosites in mice striatum (STR) treated with vehicle (VEH), an improving dose (SKF-LOW), or an impairing dose (SKF-HIGH) of SKF 38393. The phosphosites with increased intensities are marked in red and the phosphosites with lower intensities are marked in blue. b Venn Diagram of differentially expressed phosphosites in mice treated either with vehicle, an improving dose, or an impairing dose. c Volcano plot of the phosphosites belonging to the low improving dose relative to vehicle based on the average ratio of three technical replicates and p-value (− log10 p-value). Grey points represent unchanged proteins, and orange and purple represent the upregulated and downregulated proteins, respectively. The indicated proteins are discussed in the text. d GO terms overrepresented in the low improving versus vehicle comparison. GO:MF refers to Gene ontology Molecular Function, GO:BP refers to the Gene Ontology Biological processes and GO:CC refers to the Gene Ontology Cellular Components. e Volcano plot of the phosphosites belonging to the high impairing dose versus vehicle comparison according to the average ratio of three technical replicates and p-value (− log10 p-value). Grey points represent unchanged proteins, and orange and purple represent the upregulated and downregulated proteins respectively. The indicated proteins are discussed in the text. f GO terms overrepresented in the high impairing dose versus vehicle comparison. GO:MF refers to Gene ontology Molecular Function, GO:BP refers to the Gene Ontology Biological processes and GO:CC refers to the Gene Ontology Cellular Components. Differential abundance of phosphosites analysis was performed using the one way ANOVA test with a threshold of FDR < 0.05. Source data are provided in the Source Data file.