Fig. 1: IL-1 is crucial for the immune defense against C. albicans.
A Expression of Il1r1 and its ligands in whole kidney of WT mice 1 and 3 days after infection with 105 CFU C. albicans. Il1a, Il1b and Il1r1 gene expression were measured using real time PCR, and normalized to TBP expression. (n = 4/5 mice per timepoint), ud=undetectable, au=arbitrary unit. Statistical test: Two-sided multiple unpaired t tests, P < 0.05 is marked with *. P[Il1b] = 0.0246, P[Il1r1] = 0.0002. Data are presented as mean values with +/- SD. B Bodyweight loss of WT and Il1r1-/- mice after infection with 105 CFU C. albicans. (n = 3 mice per group, representative of more than 2 independent repeats), mean values +/- SD are indicated. Statistical test: Two-sided unpaired T test for Area Under Curve, P < 0.01 marked with **. P[AUC] = 0.007. C Compiled data of fungal burden in kidney, brain, liver and spleen of WT and Il1r1-/- mice, at 3 days p.i. with 105 CFU C. albicans. Data from independent experiments (number of independed repeats is indicated). Icons represent the mean of a group in each experiment, means from the same experiment are connected with a line. n = 3/5 mice per group in each experiment. Statistical test: Two-sided paired t-test, P < 0.05 marked with *, P < 0.01 marked with **, P < 0.001 marked with ***. P[kidney] = 0.0002, P[brain] = 0.0003. P[liver] = 0.0059, P[spleen] = 0.0538. D Fungal burden in kidney and brain of WT and Il1r1-/- mice 24 h after infection with 105 CFU C. albicans (n = 3 mice per group, representative of more than 2 independent repeats). Statistical test: Two-sided multiple unpaired t tests, Individual variance assumption for each organ, Holm-Sidak method alpha = 0.05. E Fungal burden in kidney, brain, liver and spleen of WT, Il1a-/-, Il1b-/- and Il1r1-/- mice, at 3 days after infection with 105 CFU C. albicans. (n = 4 mice per group, representative of 2 independent repeats) Statistical test: One-way ANOVA per organ with Tukey’s multiple comparison test. P < 0.05 marked with *, P < 0.01 marked with **, P < 0.001 marked with ***, P < 0.0001 marked with ****. F, G GMS (Modified Grocott methenamine silver) staining of kidney (F) and brain (G) sections of Il1r1+/- and Il1r1-/- mice 48 h after infection with 105 CFU C. albicans. Zoomed-in areas are marked with a square. (A representative picture from a single experiment with n = 3 mice per group is shown). Hematoxylin was used for background staining. Light microscopy is shown, no manipulations to the files were done.
