Fig. 2: Il1r1-/- mice mount an exacerbated inflammatory response.
A Levels of inflammatory cytokines and chemokines in kidney and brain lysates from WT and Il1r1-/- mice, 2.5 days after infection with 105 CFU C. albicans. n = 3/4 mice per group, mean values are indicated. Statistical test: Two-sided multiple unpaired t tests for each cytokine, Two stage step up (Benjamini, Krieger and Yekutieli), desired FDR (false discovery rate) = 1.00%. B Clustering using UMAP of flow cytometry data, obtained from kidney and brain of WT and Il1r1-/- mice, 3 days after infection with 105 CFU C. albicans. Multi dimentional data was aquired from cells stained with a 14 antibody panel and analysed in Flow-Jo using the UMAP algorithm. C Heatmaps, visualizing mean expression of markers by 23 clusters, calculated using the Phenograph algorithm on data obtained from (B). Clusters are sorted acording to similarity and further sub-gropued and anontated based on the literature. Color coding allows visualization of the cell types in the UMAP plots in (B). D, E Quantification of the cell numbers in WT vs. Il1r1-/- mice. n = 5 (WT) and n = 6 (Il1r1-/-) mice per group. (Representative of 2 independent repeat). Statistical test: Two-sided unpaired t test for each cluster group. P < 0.05 marked with *, P < 0.01 marked with **, P < 0.001 marked with ***, P < 0.0001 marked with ****.
