Fig. 4: IL-1 acts via non-hematopoietic cells in the immune defense against C. albicans.

A Reciprocal bone marrow chimeras were generated from WT and Il1r1^-/- mice, and infected with 10⁵ CFU C. albicans, 10–12 weeks after reconstitution. Left: Bodyweight loss during the infection. Right: Fungal burden at 3 days p.i.. Each dot represents an animal. (n = 5 mice per group, representative of two independent repeats) Statistical analysis was performed using one way ANOVA with Bonferroni’s multiple comparisons. P < 0.05 marked with *, P < 0.01 marked with **, P < 0.001 marked with ***, P < 0.0001 marked with ****. Data are presented as mean values with +/- SD. B Fungal burden in Il1r1^fl/fl vav^cre and Il1r1^fl/fl mice at 3 days after infection with 10⁵ CFU C. albicans. (n = 4 mice per group, two independent repeats were performed) Statistical test: Two-sided multiple unpaired t tests per organ, with no correction for multiple comparisons. C, D Quantification of GFP⁺ cells in kidney and brain of KappaBle mice, 4–5 h after i.v. injection of IL-1b or PBS as control. (n = 3 C, n = 3/4 D mice per group, data from a representative experiment of two independent repeats are shown). Gating of cells is described in supplementary Fig. 4. Statistical test: Two-sided multiple unpaired t tests, with no correction for multiple comparisons. P < 0.05 marked with *. Data are presented as mean values with +/- SEM. E Compiled data of fungal burden of Il1r1^fl/fl Pdgfb^creERT2 and Il1r1^fl/fl mice, at 3 days p.i. with 10⁵ CFU C. albicans. Inducible cre recombinase was activated by 5 consecutive i.p. injection of Tamoxifen. Both groups recieved the same treatment, and were infected 4 days after the last Tamoxifen injection. Data from 5 independent experiments are shown, (n = 3–5 mice per group). Data from the same experiment are connected with a line. Statistical test: Multiple paired t tests, with no correction for multiple comparisons. P[kidney]= 0.036784. F Quantification of myeloid cell infiltration into kidney and brain, 8 h after infection of Il1r1^fl/fl, Il1r1^fl/fl Pdgfb^creERT2 and Il1r1^-/- mice with 10⁵ CFU C. albicans. Gating according to the scheme in supplementary Fig. 1. (n = 4 mice per group). Statistical test: 2-way ANOVA with Tukey’s multiple comparisons test. P < 0.05 marked with *, P < 0.01 marked with **, P < 0.001 marked with ***. Data are presented as mean values with +/- SEM. G Quantification of myeloid cell infiltration into kidney and brain, 4 h after i.v. injection of IL-1b or PBS, into Il1r1^fl/fl and Il1r1^fl/fl Pdgfb^creERT2 mice. (n = 3 mice (Il1r1^fl/fl + Il-1b, Il1r1^fl/fl Pdgfb^creERT2 + Il-1b), n = 2 mice (Il1r1^fl/fl + PBS)). No statistical test was used, as data was not large enough. Data are presented as mean values with +/- SEM.