Fig. 5: Massive and uniform transcriptional upregulation across all kidney cell types in Il1r1^-/- mice immediately upon C. albicans infection.

Mice were infected with C. albicans (10⁵ CFU) and kidneys harvested 8 h later and processed for single nuclear RNA sequencing as described in Methods. A UMAP of annotated celltypes with barplot for abundance per condition. PT-S1 - S1 segment of proximal tubule; PT-S2 - S2 segment of proximal tubule; PT-S3 - S3 segment of proximal tubule; CTAL-PT - thick ascending limb of loop of Henle in cortex associated with proximal tubules; CTAL1 - thick ascending limb of loop of Henle in cortex 1; CTAL2- thick ascending limb of loop of Henle in cortex 2; MD - macula densa; MTAL - thick ascending limb of loop of Henle in medulla; DCT - distal convoluted tubule; DCT-CNT - intermediate between DCT and CNT; CNT - connecting tubule; PC - principal cells; ICA - type A intercalated cells of collecting duct; ICB - type B intercalated cells of collecting duct; EC - endothelial cells; MC - mesangial cells; DTL_tAL - thin descending limb of loop of Henle + thin ascending limb of loop of Henle; Pod - podocytes; Mac - macrophages; PRAT - perirenal adipocytes; B Differentially expressed (DE) genes per pairwise comparison between conditions per celltype; C Shared genesets between celltype specific DE genes for Il1r1^-/- infected (top) versus Il1r1^+/- infected (bottom) comparison; D Urea and E ACR (albumin to creatinine ratio) levels in blood of Il1r1^+/- and Il1r1^-/- mice infected with 10⁵ CFU C. albicans at indicated time points after infection (n = 4/5 mice per group). Statistical test: Two-sided multiple unpaired t tests, set P value threshold = 0.05%. P[Urea 48 h] = 0.000908, P[ACR 24 h] = 0.033929, P[ACR 48 h] = 0.000007. Data are presented as mean values with +/- SD.