Fig. 4: Nf2 disturbance led to deformed focal adhesion dynamics.
From: Nf2-FAK signaling axis is critical for cranial bone ossification and regeneration
a Confocal images of immunostaining assay showed that the extent of p-Paxillin-labeled focal adhesion and Phalloidin-labeled F-actin in Nf2 mutant MSC and control. b arrow indicated higher magnification of FA in (a). c The area of FA size was quantified using ImageJ (n = 12). d Live cell imaging analysis from transient transfection of Paxillin-GFP into primary osteoblasts showed that Paxillin-GFP-labeled focal adhesion dynamics were properly growing in control osteoblasts (enlarged magnification shown in d’ in arrow), but Paxillin-GFP-labeled focal adhesions were mainly situated in cytoplasm in Nf2 mutant osteoblasts (enlarged magnification shown in d” in arrow). e The ratio between properly developed Paxillin+ cells and total cells in Nf2 mutants was notably lower compared to the controls (n = 3). Scale bar: 10 μm in (a–c), 50 μm in (d). Data were expressed as means ± SD and each dot represents an individual biological replicate. P values were calculated by unpaired Student’s t-test with two-tailed analysis without adjustments. *p < 0.05. **p < 0.01, ***p < 0.001.
