Fig. 4: PMSK1 phosphorylation status controls starch metabolism in Chlamydomonas reinhardtii.

GAP1 transcription level (a) and starch content (b) of various PMSK1 overexpression lines synchronized to a 12/12 light dark cycle under different light qualities. Data are presented as mean ± SD (n = 3 biologically independent samples). Phases are indicated by white and gray shading in (a). Line colors indicated light qualities. Black, white light; Red, red light; Blue, blue light. In b red triangles indicate sample collection time. The dark phase is indicated by black bars above the graphs; the light phase by white, blue, or red bars, depending on the light quality used. The statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparisons tests (a) of log10 transformed mRNA data as indicated in the graphs. The statistical significance was determined using one-way ANOVA with Dunnett’s multiple comparisons tests (b). Asterisks indicated the p-values compare to WT (*p < 0.05; ***p < 0.001; ****p < 0.0001; ns, not significant). In some cases, the error bars are smaller than the data point symbols. Detailed statistical analyses are presented in the Source Data File.