Fig. 5: PHOT regulates starch metabolism via PMSK1 and GAP1 in Chlamydomonas reinhardtii.

a GAP1 transcription level and b starch content in single and double phot and pmsk1 mutants synchronized to a 12/12 light dark cycle under different light qualities. Data are represented as mean ± SD (n = 3 biologically independent samples). Line colors indicated light qualities. Black, white light; Red, red light; Blue, blue light. Red triangles indicate sample collection time. Dark phase is indicated by black bars above the graphs; the light phase by white, blue, or red bars, depending on the light quality used. Red triangles indicated samples collection time. The statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparisons tests (a) of log10 transformed mRNA data as indicated in the graphs. The statistical significance was determined using one-way ANOVA with Dunnett’s multiple comparisons tests (b). Asterisks indicated the p-values compare to WT (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant). In some cases, the error bars are smaller than the data point symbols. Detailed statistical analyses are presented in the Source Data File.