Fig. 2: The putative substrate-binding cavity of PimE.
From: Mechanistic studies of mycobacterial glycolipid biosynthesis by the mannosyltransferase PimE
a The putative active site cavity within PimE is colored in semitransparent yellow, showing its elongated, cashew-like shape and orientation relative to the membrane plane. b, c PimE viewed parallel (b) and perpendicular (c) to the membrane plane, with the elongated, cashew-shaped cavity shown in yellow. The cavity is oriented almost parallel to the membrane plane, with its two ends (Cavity End 1, CE1, and Cavity End 2, CE2) curving slightly toward the membrane. The cavity is surrounded by periplasmic loops (PL1 to PL3) and the connecting loops between TM helices 3 and 4 and TM helices 9 and 10. d–f PimE rendered in surface representation colored by hydrophobicity on a purple (very hydrophilic) to tan (very hydrophobic) scale (d) by conservation on a cyan (low conservation) to magenta (absolute conservation) scale (e) and by electrostatic potential (f) on a range of ±5 kBT/e. The putative reaction center at the central part of the cavity is marked with a dotted circle.
