Fig. 3: Rehabilitation restores synaptic input to stroke-projecting neurons. | Nature Communications

Fig. 3: Rehabilitation restores synaptic input to stroke-projecting neurons.

From: Parvalbumin interneurons regulate rehabilitation-induced functional recovery after stroke and identify a rehabilitation drug

Fig. 3

a Approach for synaptic inputs in stroke-projecting neurons. b Timeline, virus vectors, and virus injection locations in dendritic spine analysis (upper) and monosynaptic tracing (lower). The dashed lines in the brain illustration indicate future stroke sites (CFA). c Representative images showing dendritic spines (upper: scale bar 5 μm) and G-deleted rabies virus (RVdG) labeled cells (middle and lower: scale bar 1 mm). cRFA: contralesional rostral forelimb area, iRFA: ipsilesional RFA, TH: thalamus, SS: somatosensory area. d, e Spine density in layer 5 stroke-projecting neurons; basal dendrite (d), n = 19 (Sham), 20 (Sham + Rehab), 27 (Stroke) or 22 (Stroke + Rehab), apical dendrite (e), n = 15 (Sham), 15 (Sham + Rehab), 22 (Stroke) or 16 (Stroke + Rehab). Kruskal-Wallis test. 4–5 animals per group. f–h The number of RVdG-labeled cells normalized by the starter cells in the whole brain (f), RVdG injection neighbor: RFA (g), and distant brain areas (h). ORB: orbital area, CFA: caudal forelimb area, SS: somatosensory area, CLA: claustrum, AC: anterior cingulate area, CNU: cerebrum nuclei (striatum and pallidum), TH: thalamus. Kruskal-Wallis test. n = 9 (Sham), 8 (Sham + Rehab), 9 (Stroke) or 11 (Stroke + Rehab). (a, b) Created in BioRender127.

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