Fig. 4: Stroke-projecting neurons are necessary for rehab-induced functional recovery. | Nature Communications

Fig. 4: Stroke-projecting neurons are necessary for rehab-induced functional recovery.

From: Parvalbumin interneurons regulate rehabilitation-induced functional recovery after stroke and identify a rehabilitation drug

Fig. 4

a Timeline, virus vectors, and injection sites in the chemogenetic inhibition targeting stroke-projecting neurons. b Representative images of the stroke-projecting neurons with hM4D(Gi)-mCherry in the RFA layer 5. The stroke-projecting neurons express a cortical projection neuron marker, Satb2 (right), but not PV (left). scale bar 50 μm. Similar staining was confirmed in the sections from 9 mice. See supplemental material for the quantification. c, d Motor performance in the skilled reaching test (c time by group, F (15, 204) = 9.930, P < 0.0001) and the grid walk test (d time by group, F (15, 204) = 17.86, P < 0.0001). Two-way repeated measure ANOVA, Sidak’s multiple comparison test. e, f Motor performance changes induced by the chemogenetic inhibition in the skilled reaching test (e) and the grid walk test (f). Two-tailed paired t-test. (c–f) n = 11 (Sham/mCherry), 11 (Sham/hM4D), 12 (Stroke/mCherry), 13 (Stroke/hM4D), 13 (Stroke+Rehab/mCherry) or 14 (Stroke+Rehab/hM4D) Data are presented as means ± sem. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (a) Created in BioRender127.

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