Fig. 5: Rehabilitation induces synapse formation from PV interneurons to stroke-projection neurons. | Nature Communications

Fig. 5: Rehabilitation induces synapse formation from PV interneurons to stroke-projection neurons.

From: Parvalbumin interneurons regulate rehabilitation-induced functional recovery after stroke and identify a rehabilitation drug

Fig. 5

a Schematic illustration of interneuron marker labeling combined with monosynaptic tracing. PV: parvalbumin, SOM: somatostatin, 5HT3a: serotonin receptor 3a. b Representative images of RVdG labeled PV interneurons. The green channel shows the GFP labeled starter cells. Scale bar 50 μm c–e The ratio of PV (c), SOM (d), 5HT3a (e) in total local inputs. Kruskal-Wallis test, n = 6. f Schematic illustration of GRASP labeling of synapse formed by stroke-projecting neuron and PV interneuron. g Timeline, virus vectors, and injection sites in the GRASP study. h, i Representative images of GRASP labeled dendrite (H, scale bar 5 μm) and soma (I, scale bar 10 μm). j Example of reconstruction of dendritic tree and GRASP+vGAT puncta. Scale bar 30 μm. GRASP expression patterns were consistent across the staining sessions and the animals k, l The number of GRASP+vGAT puncta (k) and the proportion of GRASP positive synapse in the total vGAT positive synapse. (l) on the stroke projecting neurons. Kruskal-Wallis test, n = 26 (Sham), 26 (Sham + Rehab), 23 (Stroke) or 24 (Stroke + Rehab). (a, f, g) Created in BioRender127.

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