Fig. 7: Stroke reduces the fraction of fast-rising large amplitude IPSCs and rehabilitation restores it. | Nature Communications

Fig. 7: Stroke reduces the fraction of fast-rising large amplitude IPSCs and rehabilitation restores it.

From: Parvalbumin interneurons regulate rehabilitation-induced functional recovery after stroke and identify a rehabilitation drug

Fig. 7

a Virus injection and time course for patch-clamp recording. b Representative spontaneous EPSC traces. c, d Frequency (c), and peak amplitude (d) of spontaneous EPSC. Kruskal-Wallis test, n = 11 (Sham), 12 (Stroke), or 13 (Stroke + Rehab). e representative traces of EPSCs evoked by optogenetic stimulation targeting thalamic axons. f, g Response probability (f), and peak amplitudes (g) responding to 50 sequential optogenetic stimulations. Generalized linear mixed model (Fixed effect: Stimulation number; P < 0.0001, Group; P < 0.0001). Two-sided. Tukey’s HSD correction for multiple comparison. n = 11 (Sham), 12 (Stroke), or 13 (Stroke + Rehab). h Representative spontaneous IPSC traces. i, k Frequency (i), and peak amplitude (j) of spontaneous IPSC. Kruskal-Wallis test, n = 12 (Sham), 9 (Stroke), or 10 (Stroke + Rehab). *P < 0.05, ***P < 0.001,****P < 0.0001. k Distribution of IPSC rise rate and peak amplitude. The red dashed lines indicate the mean peak amplitude and rate of rise of all IPSC events from all recordings. The large amplitude with fast rate of rise events above both mean values (upper right quadrant) amount to 16.5%, 11.7%, and 19.7% of all IPSC events in the Sham, Stroke, and Stroke + Rehab groups, respectively. Chi-squared test; X-squared = 73.4, df = 2, P = 2.2e-16, Bonferroni post hoc test, two-sided; Sham vs Stroke: P < 0.0001, Sham vs Stroke + Rehab: P = 0.0092, Stroke vs Stroke + Rehab; P < 0.0001. Data are presented as means ± sem. (a) Created in BioRender127.

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