Fig. 1: Unassigned density regions in the reconstructions by Linares et al. contain features consistent with fragments of TMP. | Nature Communications

Fig. 1: Unassigned density regions in the reconstructions by Linares et al. contain features consistent with fragments of TMP.

From: Reply to: About proteins of a siphophage tail tip complex reverting to their pre-ejection fold after DNA ejection

Fig. 1

a Map features located along the inner wall of the baseplate hub protein (BHP) in EMD-14873. EMD-14873 is displayed after applying a local resolution filter calculated from the deposited half maps. The location of this density region coincides with that of the C-terminal fragment of the TMP (CTMP) in our map. b Radial section of the same area as in (a). The unassigned densities can be seen parallel to the inner wall of BHP. c Comparison of EMD-14873 (left) and our reconstruction of the empty bacteriophages (right). Red box: unassigned region of density shown in (a) and (b). Blue box: region in our empty bacteriophage reconstruction corresponding to CTMP. d Additional unassigned region with low-resolution density is present in EMD-14873. e Region of unassigned density in EMD-14800. Due to this region’s low resolution, it is impossible to determine what component it contains. Therefore, it cannot be ruled out that it includes the CTMP after conformational rearrangement upon binding to the FhuA receptor. The deposited unsharpened version of EMD-14800 is displayed. f Additional region with weak density observed in EMD-14800.

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