Fig. 5: Dynamic imaging of cell anatomy across all the mitosis stages.
From: Fast segmentation and multiplexing imaging of organelles in live cells
a Extended-resolution images of a U2-OS cell with its membranous structures stained by Nile Red and the nuclei structure stained by NucSpot® live dye. b–g The arrangement of intracellular structures including cytosol, nucleus, ECS, ER, nuclear membrane, nuclear reticulum, Golgi, lipid droplet, mitochondria, lysosome, early endosome, late endosome, plasma membrane, filopodia, and peroxisome at interphase, prophase, metaphase, anaphase, and telophase. The yellow arrow in (b) points from the bottom of the coverslip to the top of the cell. h The total volume analysis of cytosol and nucleus at different mitosis stages. i The volume proportion of ER, Golgi, lipid droplet, mitochondria, lysosome, early endosome, late endosome, peroxisome, nuclear membrane, nuclear reticulum, plasma membrane, and filopodia at different mitosis stages. The total volume calculates the foreground area of Nile Red fluorescence images. See Fig. S14 for detailed volume statistics of different intracellular structures. Each independent experiment representing distinct mitotic stages was replicated at least three times (n ≥ 3). Scale bar: 5 μm.
